Isolation and identification of Leishmania sp of Amblyomma naponense and Amblyomma humerale collected from Pecari tajacu, Tayassu pecari and Chelonoide denticulata

The aim of this study was to isolate and identify Leishmania from Amblyomma naponense humerale collected Pecari tajacu, Tayassu pecari Chelonoides denticulata. ticks were denticulata in Madre de Dios, Peru. viscera salivary glands the extracted, direct observations made under microscope. Samples cultured biphasic blood agar inoculated into footpads intraperitoneal hamsters. In none observations, cultures responses inoculations parasite isolated or identified. These results show basic research which serves recommend use a larger number various animals as potential reservoirs highly sensitive detection technique such polymerase chain reaction (PCR).